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Downregulation of key constituents controlling mitochondrial biogenesis in peripheral lung tissue from very severe COPD patients To evaluate if, and to what extent, the pathways regulating mitochondrial turnover (biogenesis versus breakdown of mitochondria) are affected in peripheral lung tissue from COPD patients, we first investigated the mRNA expression of molecules involved in the regulation of mitochondrial biogenesis

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- **Crackles (or rales):** - Crackles are high-pitched, rattling, or clicking sounds