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HCC38 (5000) or SUM149 cells (10,000) were cultured in 0.9 mL of 1% methylcellulose/complete DMEM medium in ultra-low adhesion 24-well dishes (Corning, Corning, NY, USA) and cells cultured for 1214 days

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African civet remains comparatively secure due to broad distribution and ecological flexibility, but local declines are consistent with increasing hunting pressure and habitat conversion

They are durable when they are made from high-quality materials