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In their study, researchers from the Division of Health Studies at the University of Waterloo, Ontario, and Physicians for a Smoke-Free Canada highlighted problems with the ISO test, where a cigarette is inserted in a machine to measure tar and nicotine levels

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In patients with smoking-related COPD, GPx is up-regulated in the epithelial lung tissue, but downregulated in blood components, such as plasma and erythrocytes [74, 75]