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Immune cell isolation from induced sputum and peripheral blood To eliminate mucus from sputum samples, the same volume of 0.1% dithiothreitol (Sigma, MO, USA) was added, the tube was shaken for 20 min at 37 C, and the mixture was filtered through a 70 m strainer

Han SG, Howatt DA, Daugherty A, Gairola CG (2012) Atherogenic and pulmonary responses of ApoE- and LDL receptor-deficient mice to sidestream cigarette smoke

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doi:10.1158/1055-9965.EPI-09-0620