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Cessation-focused visits (CPT 99406/99407): For dedicated tobacco cessation counseling encounters, the principal diagnosis should be either the underlying condition the smoking is impacting (COPD, ischemic heart disease, etc.) or for outpatient counseling visits where no such condition is being addressed a Z-code such as Z71.6 (Tobacco abuse counseling) as the encounter reason, with F17.210 sequenced as a secondary diagnosis to specify the nicotine dependence

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Cell lines Immortalized human bronchial epithelial cells (BEAS-2B) were purchased from ATCC (Manassas, VA) and maintained in Bronchial Epithelial Cell Medium (BEBM) with growth supplements (Lonza, Walkersville, MD)