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PCR was done with Pfu polymerase according manufacturers instruction (Fermentas), sequencing was done by Sequencing laboratory, Faculty of Science, Charles University in Prague, Czech Republic)

When nicotine levels are reduced in the body, withdrawal symptoms like restlessness, irritability, and anxiety lead to an increase in stress levels, and hence, the individual is compelled to smoke again

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1 2 Brandt, Allan M

This is all with a direct similarity to the experience of smoking a cigarette, making vaping products the most visually similar and reminiscent of the experience, making them highly popular if you are trying to quit smoking