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Unpaired t -tests were applied to compare two groups, three or more groups were compared with a one-way ANOVA followed by Tukeys or Dunnetts multiple comparisons test, and two-factor analyses were conducted with a mixed-effects model followed by dks or Tukeys multiple comparisons test

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Data analysis We conducted a thematic content analysis of the data, creating emergent codes under our pre-specified research questions [18]

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