Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB) and residual linear DNA was degraded by a cocktail of exonucleases containing plasmid-safe ATP-dependent DNase (Lucigen), lambda exonuclease (NEB) and exonuclease I (NEB)
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On the contrary, negative public perception and health concerns, stringent government regulations and bans, high initial costs of advanced devices, and concerns regarding youth vaping epidemic are anticipated to slow down e-cigarette and vape market penetration across the study period and beyond
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2004 Davis S, Malarcher A, Thorne S, Maurice E, Trosclair A, Mowery P: State-specific prevalence and trends in adult cigarette smoking - United States, 1998-2007
