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Immunofluorescence staining (IF) Organoids were fixed in 4% paraformaldehyde overnight at 4 C, permeabilized with 0.5% Triton X-100 for 1 h at room temperature, and incubated with H2A.X primary antibody (1:200, 9718, Cell Signaling Technology, MA, USA) in blocking buffer overnight at 4 C

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