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2.5 Tobacco yield and quality Following complete plot harvest for yield determination, tobacco leaves from each treatment were graded according to the National Standard for Flue-Cured Tobacco Grading (GB 2635-1992) and the proportions of premium-, medium-, and low-grade leaves were computed for subsequent statistical analysis ( 2.6 Index calculations To assess the soil quality index and microbial community characteristics, the soil quality index (SQI) and soil microbial index (SMI) were calculated

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