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GFAP staining intensities was measured using the Fiji-ImageJ (Media Cybernetics, Silver Spring, MD) in five randomly selected fields per coverslip (8 coverslips per culture, 6 cultures for each animal group) Staining intensity was quantified using the Corrected Total Cell Fluorescence (CTCF), calculated as: CTCF = Integrated Density(Area of selected cellMean fluorescence of background readings)

in Kurzlehrbuch Physiologie (Georg Thieme Verlag, Stuttgart, 2009)

A shell can change the local environment of QDs surface, physically separates the surface of the optically active core from its surrounding medium 53