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After decantation and centrifugation, the iron hydroxide precipitate was dissolved in 100 ml of 1 M HCl and 1 g of ascorbic acid was added to reduce Fe 3+ to Fe 2+

[32] [33] [34] Although bacteria degrade nicotine in various ways, one example of this degradation is A.nicotinovorans , a bacterium that uses the pyridine pathway to attack the 6 carbon in nicotine through hydroxylation

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