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Methods Long-term CSE exposed human bronchial epithelial cell culture BEAS-2B cells were grown in RPMI 1640 (Lonza, Walkersville, MD) supplemented with 15% heat-inactivated Fetal Bovine Serum (FBS), penicillin (100 U/ml) (Lonza) and streptomycin (100 g/ml) (Lonza) on collagen-coated plates

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1 cm), the position of plunger was fixed using pipette tips

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