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American Journal of Neurodegenerative Disease, 1 (1), 1-14

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In the Philippines, 23.8% of its citizens consume tobacco productsin the teen demographic aged 1315, 16% use tobacco products, while in the aging population (65 and up), 29.7% of men and 8.6% of women smoke

In this step, RPMI culture medium (Bio-IDEA), which is suitable for PBMCs cell culture, along with FBS and trypan blue dye, was utilized to identification of living and dead cells

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