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While rare, improper storage (like leaving it in high heat or upside down) can cause leaks

An aliquot of cells (210 4 cells) was prepared by centrifugation (Cytospin 11, Shandon Instruments, Pittsburgh, PA), air-dried, fixed in 4% paraformaldehyde in PBS, and stained using Diff-Quik (Dade Behring, Newark, NJ)

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