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RNA-seq and ssGSEA analysis of TCGA cohort and cell lines UMSCC47 (RRID: CVCL_7759) and UDSCC2 cells were harvested after acute exposure to smoke-infused media for 8 h, whereas SCC152 and SCC 154 cells were harvested following chronic smoke-infused media exposure with an additional bolus 8 h prior to isolation

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Smokers are more likely to report higher pain intensity and a lower threshold for pain, complicating the management of chronic conditions like back pain

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