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This research encompasses various dimensions, including growth, development, physiological responses, yield, and quality ( Figure 1 Effects of heat and drought stress on the growth and development of tobacco plants Severe drought stress and prolonged exposure to temperatures exceeding the optimal range can damage plant growth and development ( Tobacco plants require sufficient water throughout their entire growth period, with different stages demanding varying water levels ( 2 assimilation rate will significantly downregulate ( Role of physiological and biochemical responses during growth and development stage Photosynthesis is widely recognized as one of the most sensitive physiological processes in response to heat stress ( Additionally, heat stress directly affects the activity of respiratory enzymes, reducing respiration intensity ( 2 concentration in tobacco cultivar Yunyan 87 initially decreases, then increases, and decreases again, indicating that prolonged heat stress weakens tobacco leaf respiration ( 2 assimilation enzyme activities are the main factors causing to decline photosynthetic responses ( 2 uptake and reducing photosynthesis due to inadequate raw materials