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The membrane was visualized with an enhanced chemiluminescence kit (SuperSignal ECL kit, Thermo Scientific) and detected by ChemiLux Imager (Intas, Gttingen, Germany) and quantified using the GelPro software

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Moreover, to mitigate the influence of short-term fluctuations and ensure robustness of the results, the Average Annual Percent Change (AAPC) was employed as the primary metric for assessing trend variations across the different regions, countries, and territories