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Methods The severity of airway remodeling and inflammation was studied by analyzing alveolar enlargement, heart hypertrophy, inflammatory cells in the bronchoalveolar lavage fluid (BALF) and lung tissue and by determining the cytokine and chemokine profiles in the BALF of A/J mice exposed to cigarette smoke for 20 weeks and 8 weeks after smoking cessation

INTRODUCTION Tobacco necrosis virus A (TNVA) is an icosahedral virus that encapsidate one linear single-stranded RNA molecule ssRNA(+) and forms virions of 28 to 35 nm in diameter (Kotta-Loizou et al., 2019

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The mixture was salted out by adding MgSO 4 (4 g) along with NaCl (1 g) and vigorously vertexing for 2 min followed by centrifugation at 4200 rpm (5 min)