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The calculation for dry matter percentage is as follows: Disappearance determination and calculations The disappearance was assessed using the indicator method with 4 mol/L HCl insoluble ash as a marker ( The calculation of acid-insoluble ash is as follows: where M = mass in g of sample used for the analysis, M0 = mass in g of the empty crucible, and M1 = mass in g of the crucible and sample after incineration

Some of them employ other mechanisms to detoxify metal ions, such as intracellular accumulation, efflux or adsorption at the cell wall (Essahale et al

This is consistent with the original description of the Johnsen scoring system, which emphasizes germ cell maturation rather than morphometric parameters 31