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Column chromatography was performed on silica gel (Kieselgel 60, 70230 mesh, Merck, Darmstadt, Germany), and semi-preparative HPLC was performed using a Waters HPLC system (Waters, Milford, MA, USA) equipped with two Waters 515 pumps with a 2996 photodiode-array detector

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The activity of several involved enzymes also increase, including those that allow an influx of sulphur (as inorganic sulphate, that is converted into homocysteine as the entry step into biological organic species), which is the rate-limiting element for the biosynthesis of biological thiols [25,26]