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He C, Wang J, Zhang Z, Yang M, Li Y, Tian X et al

GdmCl-induced chemical unfolding GdmCl-induced denaturation of 0.1 g/l hPrdx6 at pH 7.0 (10 mM TrisHCl buffer) and pH 4.0 (10 mM sodium acetate buffer) was followed by measuring changes in max as a function of GdmCl concentration (0-5 M) at 20 C in Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an external constant temperature circulator/bath

Extraction of curcumin Curcumin was extracted from turmeric powder using a Soxhlet extractor with ethanol as the solvent

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In some cases a dermatologist is involved to exclude secondary causes of dark hyperpigmentation- examples include irritant contact dermatitis, allergic dermatitis & endogenous causes via endocrine dysfunction