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doi: 10.1159/000328989 74 MadeiraMHBoiaRSantosPFAmbrsioAFSantiagoAR

Realtime PCR was performed using TaqMan Universal Master Mix II, no UNG (Applied Biosystems PN 4440041) according to the protocol, using 1.33 l of undiluted cDNA per reaction and RNAspecific Taqman hydrolysis probes