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Briefly, muscles were washed in chilled PBS and immediately transferred to 30% sucrose and kept at 4 C until tissue sank to bottom

These systems in nano size offer better control of release of the drug, culminating in enhanced in vivo and/or in vitro anti-cancer effectiveness [300]

2d and S3b)

It is worth noting that genes associated with PC metabolism through the Kennedy pathway were modulated in the KO cells relative to the WT controls, and were reversed in the OE (Supplementary Fig