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Studies utilizing high-intensity ultrashort PEFs on U87 GBM cells and U87-derived neurospheres revealed, through the analysis of diverse in vitro biological endpoints and transcriptomic and bioinformatic analyses, that PEFs affects cell proliferation, differentially regulates hypoxia, inflammation, and p53/cell cycle checkpoints, significantly reduces the capacity to form new neurospheres, inhibits invasive potential, and alters the expression of stemness/differentiation genes (74)