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The slides were washed and then incubated with biotin-labeled goat anti-rabbit secondary antibody at 37 C for 30 min

Sulfatide is needed for phagocytosis with membrane ruffling and phagosome maturation We finally confirmed the significance of sulfatide in phagocytosis and phagosome maturation using cerebroside sulfotransferase (CST)-null ( Cst /- ) mice (61)