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Fe 3+ can bind to transferrin (TF) in serum and is subsequently taken up by the TF receptor 1 (TfR1), which is encoded by TFRC on the cell membrane [52]

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In brief, up to 1 million cells were resuspended in a total of 180 L freshly prepared FACS Buffer (PBS, 1% FSC, 2 mM EDTA)