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Substrates of NAT1 include aminobenzoic acid, aminosalicylic acid, the bacteriostatic antibiotics sulfamethoxazole and sulfanilamide, 2aminofluorene and caffeine (Ginsberg et al., 2009)

Given this data, it can be concluded that the regions prone to modification are located near the KKS linker on either the N-terminal or C-terminal side of the protein, depending on the modifier studied