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After incubation, cells were rinsed with saline and incubated with 100 mM CuSO 4 (Sigma-Aldrich, C1297) for 2 min

Similarly, a triple mutant E423Y/E442Q/D445N of BsGGT was also developed with enhanced acylase activity (Suzuki et al., 2010)

Mol Vis 18:26732686 Rosemberg DB, da Rocha RF, Rico EP, Zanotto-Filho A, Dias RD, Bogo MR, Bonan CD, Moreira JCF, Klamt F, Souza DO (2010) Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain

2.3.6 Number Stroop task Participants would see numbers with 14 ciphers (where each cipher was the same number, which could be 1, 2, 3, or 4, e.g., 22, 3, 444)