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non-phosphorylatable-VadK ( vadK:vadK His422Ala ) or phosphomimetic-VadK ( vadK:vadK His422Asp ) or a disrupted ATP-binding site, vadK:vadK Arg106Lys (predicted from (Ye et al, 2001) to disrupt ATP binding) and compared these strains to WT and with vadK:vadK (Basu et al, 2018)