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First, an arginine (R) at the third position is swapped for a glutamic acid (E)

Another CAT (HORVU.MOREX.r2.7HG0623480.1) increased progressively across Stages IIIII in both cultivars and served as an IIIII marker

K.KoppulaS.ChoiM.SukK

Ergothioneine exhibits longevity-extension effect in Drosophila melanogaster via regulation of cholinergic neurotransmission, tyrosine metabolism, and fatty acid oxidation

After incubation with primary antibodies, membranes were washed three times with 0.1% Tween 20 and TBST and then incubated with horseradish-peroxidase-conjugated anti-rabbit antibody (Cell Signaling #7074) or horseradish-peroxidase- conjugated anti-mouse antibody (Cell Signaling #7076) at 1:3000 dilutions for 1 h at room temperature