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10.1002/hep.20223 Hepatology 118 IasevoliF.BaroneA.BuonaguroE

doi: 10.1074/jbc.R800046200 31 ZouYLiHGrahamETDeikAAEatonJKWangWet al

The cDNA was synthesized by Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, CA, USA) and used as template for a two-step quantitative RT-PCR using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) with the following cycling conditions: 95 C for 5 min, 40 cycles at 95 C for 15 s and extension at 60 C for 30 s