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10.3969/j.issn.1004-2407.2014.02.019 83 LiZ.ZhangC.RenG.YangM.ZhuS.LiM

Similarly to what was observed in the case of GSAO and CAO ( S-nitrosoglutathione S -nitrosoglutathione (GSNO) is the S -nitrosated derivative of GSH and is thought to be a critical mediator of the downstream signaling effects of NO ( S -nitrosation or S -glutathiolation (Hogg et al., 1997

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For the index PCR, a 25 L reaction containing amplicon PCR product, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer (Thermo Fisher Scientific), and 0.5 M each of Nextera XT Index Kit v2 index primers (Illumina) was prepared