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Differences between experimental and control groups were assessed by either t test or the analysis of variance (ANOVA), as applicable, using SPSS 18.0 (SPSS, 165 Inc.)

Gel-purified chemically synthesized RNAs were radiolabelled directly using T4 Polynucleotide Kinase (PNK) (NEB, M0201) and [- 32 P] ATP (Revvity, BLU035C005MC) in T4 PNK reaction buffer (NEB, B0201S) at 37 C for 12 h, then desalted using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221) and gel-purified on a urea-polyacrylamide gel