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The membranes were blocked with 5% nonfat milk in Tris-buffered saline-Tween 20 (TBST) for 1 h at room temperature before being incubated with primary antibodies against p16, p21 and GAPDH (all from Proteintech, China) overnight at 4 C

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The pro-apoptotic members of this family, such as Bax and Bak, promote this permeabilization, releasing cytochrome c into the cytosol [221223]