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The dissociated single cells were seeded on AggreWell TM 800 plates (Stemcell Technologies, USA #34850) containing 300 microwells per well, pretreated with AggreWell rinsing solution (Stemcell Technologies, USA #07010), according to the users manual

The resulting mixture is maintained under conditions appropriate for ligation of the two fragments

The most important outcome measure was symptom relief, followed by objective measures such as vibration perception threshold (VPT) and nerve conduction

Treatments were administered with or without 300 ng/ml Doxycycline, 5 M A, 350 M BSO, 5 M DMF, and 0.1 M Fer-1, as indicated in the culture medium