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AhR serves as an anti-inflammatory factor in macrophages that regulates AhR-dependent gene expression pathways (Phelan et al., 1998

The inclusion bodies were washed with 25 ml of wash buffer (100 mM Tris pH 8.0, 2 mM EDTA and 0.01% v/v deoxycholate), sonicated and pelleted by centrifugation