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[ 33 ] Despite the overall decline in adolescent cigarette use across America over the past few decades, rates of electronic cigarettes have been on the rise for American youth

After the addition of 100 L of internal standard (d 4 -nicotine in acetonitrile), 400 L ammonia (5 %) and 10 mL acetonitrile, the mixture was shaken thoroughly (15 min) and ultra-sonified (15 min)