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Confocal microscopy to measure airway epithelial cell layer integrity To determine the effect of CSE on epithelial intercellular adhesion, coverslips were coated with poly-L-lysine solution (Sigma, MO, USA) for 5 min at RT, washed with PBS, and dried for 1 h at 60 C

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Therefore, women who smoke are more likely to become pregnant with chromosomal problems in the fetus, miscarriage, or birth defects